CyOFP1 EVs
Orange fluorescent protein loaded into EVs
Reference X134
CyOFP1 EVs are bioengineered EVs, loaded with CyOFP1 cyan-excitable orange fluorescent protein in their lumen using Ciloa’s pilot peptide technology, which does not overexpress surface proteins.
CyOFP1 EVs are produced in human embryonic kidney (HEK) 293T stable cell line and purified according to in-EV purification process, combining Tangential Flow Filtration and chromatography. Pure CyOFP1 EVs are subjected to standardised quality controls showing the total protein content, the number of particles, the EV size distribution, the presence of internal EV markers and the absence of cell protein contaminants. CD81 surface marker presence was revealed by a direct ELISA on pure CyOFP1 EVs. Cellular expression of CyOFP1, its sorting within EVs, and fluorescence measurement are given.
Strengths of CyOFP1 EVs
o  Purity: in-EV standardised purification process combining Tangential Flow Filtration (TFF) and chromatography allows to obtain highly purified EVs.
o  Quality: CyOFP1 EVs have been characterised using in-EV standardised quality controls.
o  Storage: CyOFP1 EVs are in a simple buffer and are stable at +4°C for several months.
o  Performance: CyOFP1 EVs are stably-fluorescent EVs.
o  Many applications: CyOFP1 EVs can be used as an internal standard for data normalisation, for EV instrument calibration or as a reference positive control in cell fluorescence imaging.
| Total protein quantity of pure EVs | 30 µg |
| Particle number (Nano-flow cytometry) | ≥ 1010 particles |
| Particle size (Nano-flow cytometry) | Median size of 60-70 nm |
| Source | HEK293T cells |
| Buffer | PBS 1X – BSA 10 µg/mL |
| Storage | Store sterile at +4°C up to 6 months |
Schematic representation of CyOFP1 EVs
Created using biorender.com
CyOFP1 EVs fluorescence intensity measurement using a CLARIOstar Plus plate reader (BMG Labtech). (n=3, SD).