EGFP EVs
Green fluorescent protein loaded into EVs
Reference X133
EGFP EVs are bioengineered EVs, loaded with EGFP, enhanced green fluorescent protein in their lumen using Ciloa’s pilot peptide technology, which does not overexpress surface proteins.
EGFP EVs are produced in human embryonic kidney (HEK) 293T stable cell line and purified according to in-EV purification process, combining Tangential Flow Filtration and chromatography. Pure EGFP EVs are subjected to standardised quality controls showing the total protein content, the number of particles, the EV size distribution, the presence of internal EV markers and the absence of cell protein contaminants. CD81 surface marker presence was revealed by a direct ELISA on pure EGFP EVs. Cellular expression of EGFP, its sorting within EVs, and fluorescence measurement are given. EV luminal localisation is demonstrated.
Strengths of EGFP EVs
o Purity: in-EV standardised purification process combining Tangential Flow Filtration (TFF) and chromatography allows to obtain highly purified EVs.
o Quality: EGFP EVs have been characterised using in-EV standardised quality controls.
o Storage: EGFP EVs are in a simple buffer and are stable at +4°C for several months.
o Performance : EGFP EVs are stably-fluorescent EVs.
o Many applications: EGFP EVs can be used as an internal reference for data normalisation, EV instrument calibration or as a positive control for EV quality control in cell fluorescence imaging.
Specifications
| Total protein quantity of pure EVs | 50 µg |
| Particle number (Nano-flow cytometry) | ≥ 1010 particles |
| Particle size (Nano-flow cytometry) | Median size of 60-70 nm |
| Source | HEK293T cells |
| Buffer | PBS 1X – BSA 10 µg/mL |
| Storage | Store sterile at +4°C up to 6 months |
Schematic representation of EGFP EVs
Created using biorender.com
EGFP EVs fluorescence intensity measurement using a fluorometer
EGFP fluorescence on cells. Imaging was performed on a confocal microscope, after incubation of cells with EGFP EVs