EV-Down CM
Concentration kit for extracellular vesicles from cell culture conditioned media
Reference K214
EV-Down CM kit allows the fast isolation and concentration of non-purified extracellular vesicles from cell culture media.
Description
An easy and quick method to concentrate EVs from cell culture media
EV-Down CM kit allows the fast isolation of extracellular vesicles (EVs) from cell culture media, without requiring ultracentrifugation. Cell culture medium containing EVs is incubated overnight with a reagent that captures water molecules, leading to the precipitation of EVs. By low-speed centrifugations, EVs are pelleted and can be resuspended in the desired buffer, in the desired volume.
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Isolated EVs can be used for several downstream applications like protein quantification, Western blot, functional assays or can be further purified using chromatography.
Schematic steps for implementing the EV-Down CM kit.
Specifications
EV-Down CM kit is designed for the concentration of non-purified extracellular vesicles, produced in culture media containing or not foetal bovine serum. EVs from serum-free culture media can also be concentrated but the efficacy might be lower. We recommend to test the efficiency of EV isolation with your own EV production medium.
Strengths of EV-Down CM
o  Quick
o  Simple to use
o  Ready to use
o  No specific equipment required
Additional information
| Â | K214_10 | K214_50 | K214_100 |
| Â Size | For 10 mL of sample | For 50 mL of sample | For 100 mL of sample |
| Â Downstream application | Protein quantification, functional assays, Western Blot, EV counting and sizing, purification | ||
| Â Shelf life | 6 months | ||
Data and examples
EVs produced in three culture media, classically used for EV production, were isolated using EV-Down CM kit and analysed by Western blot using EV-ID kit (in-EV, Ref. K211). EV markers such as Alix, Syntenin-1 and CD81 were detected in all three EV preparations. Calnexin, a cellular contamination marker, was tested to assess EV purity.
Western blot on EVs from 3 different culture media, concentrated using EV-Down CM kit.