HEK293T Standard EVs
Standard EVs from HEK293T cells
Reference X130
Standard EVs can be used as an internal reference for data normalisation, EV instrument calibration or as a positive control for EV quality control assessment.
Standard EVs are produced in human embryonic kidney (HEK) 293T cell line and purified according to in-EV purification process, combining Tangential Flow Filtration and chromatography. Pure Standard EVs are subjected to standardised quality controls showing the total protein content, the number of particles, the EV size distribution, the presence of internal EV markers and the absence of cell protein contaminants. CD81 surface marker presence is revealed by a direct ELISA on pure Standard EVs.
Strengths of Standard EVs
o  Purity: in-EV standardised purification process combining Tangential Flow Filtration (TFF) and chromatography allows to obtain highly purified EVs.
o  Quality: Standard EVs have been characterised using in-EV standardised quality controls.
o  Storage: Standard EVs are in a simple buffer and are stable at +4°C for several months.
o  Native: Standard EVs have no specific targets on their surface, allowing them to be used as negative control in functional in vitro and in vivo assays.
o  Many applications: Standard EVs can be used as an internal standard for data normalisation, for EV instrument calibration or as a reference positive control for EV quality control assessment.
| Total protein quantity of pure EVs | 50 µg | 100 µg |
| Particle number (Nano-flow cytometry) | ≥ 1010 particles | ≥ 2 x 1010 particles |
| Particle size (Nano-flow cytometry) | Median size of 60-70 nm | |
| Source | HEK293T cells | |
| Buffer | PBS 1X – BSA 10 µg/mL | |
| Storage | Store sterile at +4°C up to 12 months | |
Schematic representation of Standard EVs
Created using biorender.com
Use of Standard EVs as a positive control for the detection of EV markers
Use of Standard EVs as a reference in CD81 surface marker detection by a direct ELISA on pure EVs.
Using such a direct ELISA with these Standard EVs and your own EV batches allows i) a relative quantification and ii) an evaluation of the purity of your own EVs.
Use of Standard EVs as a negative control in EV fluorescence quantification assay (n=3, SD)