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EV-Loc
€500.00
EV-ID
€500.00
Tax excluded
Multi-species EV characterisation kit for Western blot analysis
Mutli-Species EV characterisation kit for Western blot analysis
EV-ID kit allows to characterise EVs by detecting specific EV markers and a cellular contamination marker by Western blot analysis. EV characterisation after isolation is recommended to confirm that the isolated particles are EVs and not products of cell fragmentation or other contaminants, usually present in biological samples.
EV-ID kit allows the detection of 2 surface EV markers, CD9 and CD81 and 2 internal EV markers, Alix and Syntenin-1. Moreover, EV-ID kit includes the detection of Calnexin, a cellular contamination marker, that must be absent in the pure EV preparations.
Protocol steps
- EV resuspension in Laemmli buffer
- EV denaturation
- SDS PAGE electrophoresis and transfer
- Incubation with primary antibodies
- Incubation with secondary antibodies
- HRP revelation
Specifications
| K211_2 | K211_5 | K211_10 | |
| Size | For 2 full characterisations | For 5 full characterisations | For 10 full characterisations |
| Kit content |
Internal controls (EV lysate and cell extract) Laemmli buffer for EV resuspension Primary antibodies (Alix, Calnexin, CD9, CD81 and Syntenin-1) Secondary antibodies (HRP conjugated) |
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| Downstream application | Western blot | ||
| Shelf life | At least 3 months at -20°C | ||
Strengths of EV-ID
- Kit for the full characterisation of EVs by Western blot, including 2 internal markers and 2 surface markers.
- Assessment of EV purity by providing detection of Calnexin, a representative cell contaminant.
- Positive controls also provided.
K211_2
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