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EV-ID
€500.00
EV-Loc
€500.00
Tax excluded
Multi-species EV cargo localisation kit for western blot analysis
The way to determine the localisation of a protein of interest associated with EVs
EV-Loc kit allows to determine the localisation (i.e. internal or at the surface) of proteins associated with extracellular vesicles (EVs). Thanks to the lipid bilayer, internal proteins are protected from digestion by proteases while surface proteins are not protected. By treating with proteases intact or previously permeabilised EVs, it is possible to determine the localisation of proteins which are known to be associated with EVs.
Following digestion assay, EV samples are analysed by SDS-PAGE and Western blot. In this kit, antibodies against CD81 and Alix are used to reveal surface and internal EV markers, respectively.
Protocol steps
- EV treatment
- EV denaturation
- SDS-PAGE gel electrophoresis and transfer
- Incubation with primary antibodies
- Incubation with secondary antibodies
- HRP revelation
Specifications
| K213_2 | K213_5 | |
| Size | For 2 samples | For 5 samples |
| Downstream application | Western blot | |
| Shelf life | At least 3 months at -20°C | |
Strengths of EV-Loc
- Unique kit for the inside/outside precise localisation of a protein associated with EVs.
- Works on EVs with different levels of purity (enriched EVs, pure EVs or extra pure EVs).
- Easy to use.
K213_2
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