EV-Down Milk

An easy and quick method to concentrate EVs from milk

EV-Down Milk kit allows the fast isolation of extracellular vesicles (EVs) from milk, without requiring ultracentrifugation. Milk containing EVs is skimmed, depleted of its casein and then incubated overnight with a reagent that captures water molecules, leading to the precipitation of EVs. By low-speed centrifugations, EVs are pelleted and can be resuspended in the desired buffer, in the desired volume.

Isolated EVs can be used for several downstream applications like protein quantification, particle counting, Western blot, functional assays or can be further purified using chromatography.

Protocol steps

• Milk skimming

• Casein precipitation

• Milk filtration

• Overnight incubation with reagents for EV precipitation

• Low speed centrifugations for EV pelleting

• EV resuspension

Specifications

Strengths of EV-Down Milk

• Robust kit, components fine-tuned to obtain high EV recovery yield and minimal protein contamination.

• Obtention of concentrated EVs to perform direct analysis without additional purification: Western blot, functional assays, …

• No need of ultracentrifuge, only low-speed centrifuge equipment required.