Labelled EVs
Highly pure labelled EVs
Reference X130
Highly pure labelled EVs loaded with fluorescent or luminescent proteins.
Our Labelled EVs are produced by human embryonic kidney (HEK) 293T stable cell line and purified according to in-EV purification process, combining Tangential Flow Filtration and chromatography.
Pure Labelled EVs are subjected to standardised quality controls showing the total protein content, the number of particles, the EV size distribution, the presence of internal EV markers and the absence of cell protein contaminants. CD81 surface marker presence is revealed by a direct ELISA on pure Labelled EVs.
The presence of the labelled protein is shown and EV fluorescence or luminescence is measured.
Fluorescent or luminescent proteins are loaded into the lumen of EVs using Ciloa technology, without overexpression or modification of natural EV proteins.
Proteins of interest are loaded into EV lumen thanks to the fusion with:
A peptide for internal membrane anchoring (Anchoring Peptide, AP)
A peptide for specific sorting into EVs (Pilot Peptide, PP)
By this way, several EVs have been developed, loaded with:
mCerulean (blue fluorescent protein)
EGFP (green fluorescent protein)
CyOFP1 (orange fluorescent protein)
NanoLuc® (luminescent protein)
