EV-Down Milk
Concentration kit for extracellular vesicles from milk
Reference K216
EV-Down Milk kit allows the fast isolation and concentration of non-purified extracellular vesicles (EVs) from fresh Milk.
EV-Down Milk kit allows the fast isolation of extracellular vesicles (EVs) from milk, without requiring ultracentrifugation. Milk containing EVs is skimmed, depleted of its casein and then incubated overnight with a reagent that captures water molecules, leading to the precipitation of EVs. By low-speed centrifugations, EVs are pelleted and can be resuspended in the desired buffer, in the desired volume.
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Isolated EVs can be used for several downstream applications like protein quantification, particle counting and sizing, Western blot, functional assays or can be further purified using chromatography.
Schematic steps for implementing the EV-Down Milk kit.
Specifications
EV-Down Milk kit is designed for the concentration of non-purified extracellular vesicles, produced in milk. EV-Down Milk has so far been tested on cow, goat, mare and ewe milks, as well as on breast milk. Milk has to be fresh and not pasteurised to recover high quality EVs.
Strengths of EV-Down CM
o  Quick
o  Simple to use
o  Ready to use
o  No specific equipment required
Additional information
| Â | K216_5 | K216_10 |
| Â Size | For 5 x 5 mL of milk | For 5 x 10 mL of milk |
| Â Downstream application | Protein quantification, functional assays, Western Blot, EV counting and sizing, purification | |
| Â Shelf life | 6 months | |
Data and examples
EVs from breast milk were isolated using EV-Down Milk kit (in-EV, Ref. K216) and analysed by Western blot. Alix and CD81 were followed as EV-specific markers.
EVs from livestock (goat, mare and cow) milks were isolated using EV-Down Milk kit (in-EV, Ref. K216) and analysed by Western blot. Alix, Syntenin-1, TSG101 and CD81 were followed as EV-specific markers.
