Description

Nluc EVs, the new stable luminescent generation of EVs

Nluc EVs are produced by human embryonic kidney (HEK) 293T cells and purified according to in-EV purification process. These pure EVs are characterised using standardised quality controls. NanoLuc® luminescent protein is loaded into the lumen of EVs using Ciloa technology, which does not overexpress or modify natural EV proteins.

Nluc EVs are interesting tools that can be used:

  • As an internal standard to assess the effectiveness of EV isolation methods

  • To evaluate EV purification performance

  • In cell capture assays or in vivo for biodistribution studies

Specifications

Total protein quantity of pure EVs 50 µg
Particle number (Nano-flow cytometry) ≥ 1E+10 particles
Particle size (Nano-flow cytometry) Median size of 60-70 nm
Source HEK293T cells
Buffer PBS 1X – BSA 10 µg/mL
Storage Store sterile at +4°C up to 6 months

Strengths of Nluc EVs

  • Extra pure EVs, fully characterised using standardised quality controls.

  • Bioengineered EVs using Ciloa technology, no overexpression or modification of natural EV proteins.

  • Storage at +4°C, in a simple buffer, for several months when kept sterile.

NanoLuc® is a registered trademark of Promega Corporation. NanoLuc® Technology is licensed from Promega Corporation.

Product Details
X132_50

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