Principle
One way to produced labelled extracellular vesicles (EVs) is to bioengineer the producing cells by using DNA. in-EV DNA can be used to transfect cells that will naturally produce labelled EVs in the culture medium. Transient transfections can be performed for fast screening and small scale productions. Transfections to establish stable cell lines are also possible thanks to zeocin resistance.
3 DNA are available for 3 distinct targeting of mCerulean (blue fluorescent protein) into EVs:
- mCerulean-PP: mCerulean flanked by 2 peptides for internal membrane anchoring and sorting into EVs
- CD63-mCerulean: mCerulean fused with CD63 for EV targeting
- CD81-mCerulean: mCerulean fused with CD81 for EV targeting
mCerulean: blue fluorescent protein | λexc = 433 nm ; λem = 475 nm
Specifications
| DNA quantity | 10 µg |
| DNA concentration | 0,5 mg/mL |
| Buffer | TNE 1X, sterile |
| Storage | Store sterile at -20°C, up to 1 year |
Strengths of DNA for EV-Labelling with mCerulean protein
- Strong promoter
- Ampicillin resistance
- Zeocin resistance