New application note

Quantitative analysis of EV recovery from conditioned cell culture media using EV-Down CM kit

in-EV is delighted to share the results of its collaborative study with BMG LABTECH, highlighting i) the interest of Nluc EVs as tracers for EV isolation and ii) the performance of EV-Down CM kit to isolate EVs from conditioned culture media.

Methodology

• Use of bioengineered EVs, loaded with the NanoLuc® luciferase, as luminescent tracers
• Luminescence quantification performed using the CLARIOstar® Plus high sensitivity multimode plate reader
• Comparison of EV recovery after 2 h or overnight incubation
• EV isolation performed on several culture media

Results

• Efficient recovery of EVs using EV-Down CM kit
• Stable luminescent signal in accordance with the expected concentrations
• High reproducibility

       > EV-Down CM kit is a simple, efficient and rapid solution (without ultracentrifugation) for researchers isolating EVs from conditioned culture media

       > Nluc EVs are powerful tracers for tracking EVs during isolation steps and to determine recovery rates

Application note is available here.

Thanks BMG LABTECH for this constructive technical collaboration!

Illustration picture used with permission from BMG LABTECH, taken from Application Note 406, © BMG LABTECH