EV-Loc

EV-Loc kit allows to determine the localisation (i.e. internal or at the surface) of proteins associated with extracellular vesicles (EVs) by Western blot analysis.

Thanks to the lipid bilayer, internal proteins are protected from digestion by proteases while surface proteins are not protected. By treating with proteases intact or previously permeabilised EVs, it is possible to determine the localisation of proteins which are known to be associated with EVs.

Following digestion assay, EV samples are analysed by SDS-PAGE and Western blot. In this kit, antibodies against CD81 and Alix are used to reveal surface and internal EV markers, respectively.