Principle
One way to produced labelled extracellular vesicles (EVs) is to bioengineer the producing cells by using DNA. in-EV DNA can be used to transfect cells that will naturally produce labelled EVs in the culture medium. Transient transfections can be performed for fast screening and small scale productions. Transfections to establish stable cell lines are also possible thanks to zeocin resistance.
3 DNA are available for 3 distinct targeting of EGFP (enhanced green fluorescent protein) into EVs:
- EGFP-PP: EGFP flanked by 2 peptides for internal membrane anchoring and sorting into EVs
- CD63-EGFP: EGFP fused with CD63 for EV targeting
- CD81-EGFP: EGFP fused with CD81 for EV targeting
EGFP (Enhanced Green Fluorescent Protein) | λexc = 488 nm ; λem = 507 nm
Specifications
| DNA quantity | 10 µg |
| DNA concentration | 0,5 mg/mL |
| Buffer | TNE 1X, sterile |
| Storage | Store sterile at -20°C, up to 1 year |
Strengths of DNA for EV-Labelling with EGFP protein
- Strong promoter
- Ampicillin resistance
- Zeocin resistance